Routine drinking milk proven to improve health conditions, including preventing osteoporosis and reducing the risk of diabetes and metabolic syndrome. However, recent studies from Sweden managed to find another incredible benefits of milk that is preventing the development of colon cancer.
A group of researchers from the University of Lund found that one protein in milk called lactoferricin4-14 (Lfcin4-14) can reduce the rate of cell growth of colon cancer significantly from time to time. You do this by extending the period of the cell cycle prior to the cancer cell chromosomes replicate themselves.
Not only that, Lfcin4-14 is also known to reduce DNA damage to cells of colon cancer were exposed to ultraviolet light (UV).
“Earlier, we suspect that the extension of the cell cycle in cells of colon cancer as part of the treatment with Lfcin4-14 may provide additional time for DNA repair process,” said one researcher, Professor Stina Oredsson from the Department of Biology, University of Lund.
“But recent studies we know that the DNA damage caused by UV rays can also be reduced in the cells of colon cancer that interfered with protein Lfcin4-14. Though the percentage of the decrease is small but can be quite significant,” he continued.
Initially researchers conducted experiments with cells exposes colon cancer using UV light causes DNA damage and regenerate the cells. While grown, the cells were first given protein Lfcin4-14 while the other group was not given protein.
Afterwards, the researchers evaluated DNA damage in both groups of cells by using a technique called comet assay. Why is it called comet assay? Because once it is processed, cells with damaged DNA is shaped like a comet with a tail and a complete comparison of the intensity of the ‘comet’ with a ‘tail’ indicates the number of damaged DNA.
As a result, a study published in the Journal of Dairy Science reveals that UV exposure causes an increase in the number of ‘comet’, otherwise intervention Lfcin4 protein-14 reduces the number of ‘comet’ in cells exposed to UV light.
To understand the mechanisms used Lfcin4-14 while reducing DNA damage, the researchers evaluated the levels of several proteins involved in cell cycle progression, DNA repair and cell death.
From there, researchers found an increase in the folds of endonuclease-1, a protein associated with DNA synthesis; decreased lymphoma X protein associated with B-cell lymphoma 2 (Bcl-2) are involved in the process of cell death, as well as decreased levels of ?-H2AX indicating DNA repair is more efficient.
“These changes support our hypothesis that treatment with a protein Lfcin4-14 can enhance DNA repair mechanisms,” said Dr.Oredsson as reported by Medindia on Friday (05/10/2012)
Dr. Oredsson noted that in general the cancer cells had defects in DNA repair mechanisms. That is why Lfcin4-14 may provide a greater effect on normal cells than cancer cells.
“Our data suggest that the impact of extending Lfcin4-14 in the cell cycle can contribute to the prevention of the growth of the cancer cells to normal cells,” he concluded.